Comparison of five commercially available immunochromatographic tests for the detection of norovirus in faecal specimens

K. Vyas; C. Atkinson; D. A. Clark; D. Irish

doi:10.1016/j.jhin.2015.06.013

Comparison of five commercially available immunochromatographic tests for the detection of norovirus in faecal specimens

K. Vyas
, C. Atkinson
, D. A. Clark
, D. Irish

Royal Free London NHS Foundation Trust
Barts Health NHS Trust

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Five CE-marked immunochromatography assay kits for the rapid detection of norovirus were compared with the reference method of real-time polymerase chain reaction (PCR) for the detection of acute norovirus infection. The sensitivity of the assays ranged between 23% and 59% with specificity being 75-100% when compared with real-time PCR. The data suggest that a reactive immunochromatographic assay indicates norovirus infection. However, the sensitivity of the assay would need to be significantly improved to be suitable for routine diagnostic purposes or as an alternative to laboratory analysis for near-patient testing.

Original language	English
Article number	4589
Pages (from-to)	176-178
Number of pages	3
Journal	Journal of Hospital Infection
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/j.jhin.2015.06.013
Publication status	Published - 1 Oct 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 The Healthcare Infection Society.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Immunochromatographic assays
Near-patient testing
Norovirus
Real-time PCR
Sensitivity
Specificity

Access to Document

10.1016/j.jhin.2015.06.013

Cite this

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title = "Comparison of five commercially available immunochromatographic tests for the detection of norovirus in faecal specimens",

abstract = "Five CE-marked immunochromatography assay kits for the rapid detection of norovirus were compared with the reference method of real-time polymerase chain reaction (PCR) for the detection of acute norovirus infection. The sensitivity of the assays ranged between 23\% and 59\% with specificity being 75-100\% when compared with real-time PCR. The data suggest that a reactive immunochromatographic assay indicates norovirus infection. However, the sensitivity of the assay would need to be significantly improved to be suitable for routine diagnostic purposes or as an alternative to laboratory analysis for near-patient testing.",

keywords = "Immunochromatographic assays, Near-patient testing, Norovirus, Real-time PCR, Sensitivity, Specificity",

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AU - Vyas, K.

AU - Atkinson, C.

AU - Clark, D. A.

AU - Irish, D.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Five CE-marked immunochromatography assay kits for the rapid detection of norovirus were compared with the reference method of real-time polymerase chain reaction (PCR) for the detection of acute norovirus infection. The sensitivity of the assays ranged between 23% and 59% with specificity being 75-100% when compared with real-time PCR. The data suggest that a reactive immunochromatographic assay indicates norovirus infection. However, the sensitivity of the assay would need to be significantly improved to be suitable for routine diagnostic purposes or as an alternative to laboratory analysis for near-patient testing.

AB - Five CE-marked immunochromatography assay kits for the rapid detection of norovirus were compared with the reference method of real-time polymerase chain reaction (PCR) for the detection of acute norovirus infection. The sensitivity of the assays ranged between 23% and 59% with specificity being 75-100% when compared with real-time PCR. The data suggest that a reactive immunochromatographic assay indicates norovirus infection. However, the sensitivity of the assay would need to be significantly improved to be suitable for routine diagnostic purposes or as an alternative to laboratory analysis for near-patient testing.

KW - Immunochromatographic assays

KW - Near-patient testing

KW - Norovirus

KW - Real-time PCR

KW - Sensitivity

KW - Specificity

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JF - Journal of Hospital Infection

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ER -

Comparison of five commercially available immunochromatographic tests for the detection of norovirus in faecal specimens

Abstract

Bibliographical note

UN SDGs

Keywords

Access to Document

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