Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles

Katherine N. Ward; Hoe Nam Leong; Elisabeth P. Nacheva; Julie Howard; Claire E. Atkinson; Nicholas W.S. Davies; Paul D. Griffiths; Duncan A. Clark

doi:10.1128/JCM.44.4.1571-1574.2006

Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles

Katherine N. Ward, Hoe Nam Leong, Elisabeth P. Nacheva, Julie Howard, Claire E. Atkinson, Nicholas W.S. Davies, Paul D. Griffiths, Duncan A. Clark

Research output: Contribution to journal › Article › peer-review

188 Citations (Scopus)

Abstract

Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log₁₀ copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log₁₀ copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.

Original language	English
Pages (from-to)	1571-1574
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	4
DOIs	https://doi.org/10.1128/JCM.44.4.1571-1574.2006
Publication status	Published - Apr 2006
Externally published	Yes

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10.1128/JCM.44.4.1571-1574.2006

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Ward, K. N., Leong, H. N., Nacheva, E. P., Howard, J., Atkinson, C. E., Davies, N. W. S., Griffiths, P. D., & Clark, D. A. (2006). Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles. Journal of Clinical Microbiology, 44(4), 1571-1574. https://doi.org/10.1128/JCM.44.4.1571-1574.2006

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abstract = "Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log10 copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log10 copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.",

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AU - Ward, Katherine N.

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AU - Howard, Julie

AU - Atkinson, Claire E.

AU - Davies, Nicholas W.S.

AU - Griffiths, Paul D.

AU - Clark, Duncan A.

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AB - Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log10 copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log10 copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.

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Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles

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