Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles

Katherine N. Ward; Hoe Nam Leong; Elisabeth P. Nacheva; Julie Howard; Claire E. Atkinson; Nicholas W.S. Davies; Paul D. Griffiths; Duncan A. Clark

doi:10.1128/JCM.44.4.1571-1574.2006

Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles

Katherine N. Ward
, Hoe Nam Leong
, Elisabeth P. Nacheva
, Julie Howard
, Claire E. Atkinson
, Nicholas W.S. Davies
, Paul D. Griffiths
, Duncan A. Clark

University College London
Division of Infection and Immunity
University College London Hospitals NHS Foundation Trust
Barts Health NHS Trust

Research output: Contribution to journal › Article › peer-review

195 Citations (Scopus)

Abstract

Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log₁₀ copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log₁₀ copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.

Original language	English
Pages (from-to)	1571-1574
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	4
DOIs	https://doi.org/10.1128/JCM.44.4.1571-1574.2006
Publication status	Published - Apr 2006
Externally published	Yes

Access to Document

10.1128/JCM.44.4.1571-1574.2006

Cite this

Ward, K. N., Leong, H. N., Nacheva, E. P., Howard, J., Atkinson, C. E., Davies, N. W. S., Griffiths, P. D., & Clark, D. A. (2006). Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles. Journal of Clinical Microbiology, 44(4), 1571-1574. https://doi.org/10.1128/JCM.44.4.1571-1574.2006

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title = "Human herpesvirus 6 chromosomal integration in immunocompetent patients results in high levels of viral DNA in blood, sera, and hair follicles",

abstract = "Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log10 copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log10 copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.",

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year = "2006",

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AU - Ward, Katherine N.

AU - Leong, Hoe Nam

AU - Nacheva, Elisabeth P.

AU - Howard, Julie

AU - Atkinson, Claire E.

AU - Davies, Nicholas W.S.

AU - Griffiths, Paul D.

AU - Clark, Duncan A.

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AB - Six immunocompetent patients with human herpesvirus 6 (HHY-6) chromosomal integration had HHV-6 and β-globin DNA quantified in various samples by PCR. The mean HHV-6 DNA concentration (log10 copies/milliliter) in blood was 7.0 (≥1 HHV-6 DNA copies/leukocyte), and in serum it was 5.3 (≥1 HHV-6 DNA copies/lysed cell). The mean HHV-6 DNA load (log10 copies)/hair follicle was 4.2 (≥1 copies/hair follicle cell), demonstrating that viral integration is not confined to blood cells. The characteristically high HHV-6 DNA levels in chromosomal integration may confound laboratory diagnosis of HHV-6 infection and should be given due consideration.

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