Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA

K Mehta

doi:10.1007/978-1-4939-9463-2_4

Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA

K Mehta

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Citations (Scopus)

Abstract

Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.

Original language	English
Title of host publication	Redox-Mediated Signal Transduction: Methods and Protocols, Methods in Molecular Biology, vol. 1990
Publisher	Springer
Edition	1
DOIs	https://doi.org/10.1007/978-1-4939-9463-2_4
Publication status	Published - 31 May 2019
Externally published	Yes

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abstract = "Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.",

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AB - Enzyme linked immunosorbent assay (ELISA) is a widely used technique for the measurement of antigens and antibodies alike. We describe here procedures, indirect ELISA and sandwich ELISA for the detection of 4-hydroxynonenal protein adducts. These adducts are stable compounds formed within cells and bodily fluids under conditions of oxidative stress. They can act as sensitive biomarkers of oxidative stress and are directly linked to disease pathology.

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Measurement of 4-hydroxynonenal (4-HNE) protein adducts by ELISA

Abstract

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