Semiautomatic evaluation of crypt architecture and vessel morphology in confocal microendoscopy: Application to ulcerative colitis

E. Veronese, E. Poletti, A. Buda, G. Hatem, S. Facchin, G. C. Strurniolo, Enrico Grisan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

Neoangiogenesis plays a central role in both the initiation and perpetuation of the inflammatory response during chronic intestinal inflammation. However, limited data is available on the microvascular and crypt architecture during remission phases. In this study we have evaluated the intestinal mucosa of UC patients in clinical and endoscopic remission by probe-based confocal endomicroscopy (p-CLE) and quantified microvessel tortuosity and crypt architecture by a semiautomated analysis. The system firstly automatically identifies the crypts visible in the images, and estimates the graph connecting their centers in order to estimate the crypt architecture. Hyperfluorescence in the peri-crypt region is evaluated, and leakge is automatically detected. Length and tortuosity of the manually inserted vessels assess the vascular state. 6 patients with active UC and 2 with inactive UC were evaluated acquiring 19 set of p-CLE images with matched biopsies, showing significant differences in crypt architecture, vessel morphology and hyperfluorescence patterns.

Original languageEnglish
Title of host publication13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013 - MEDICON 2013
PublisherSpringer Verlag
Pages435-438
Number of pages4
Volume41
ISBN (Print)9783319008455
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013 - Seville, Spain
Duration: 25 Sept 201328 Sept 2013

Publication series

NameIFMBE Proceedings
Volume41
ISSN (Print)1680-0737

Conference

Conference13th Mediterranean Conference on Medical and Biological Engineering and Computing 2013, MEDICON 2013
Country/TerritorySpain
CitySeville
Period25/09/1328/09/13

Keywords

  • Automatic segmentation
  • Confocal laser endomicroscopy
  • Crypts
  • Tortuosity
  • Ulcerative colitis
  • Vessel

Cite this