UBE2S is associated with malignant characteristics of breast cancer cells

Akter Khondker Ayesha; Toshinori Hyodo; Eri Asano; Naoki Sato; Mohammed A. Mansour; Satoko Ito; Michinari Hamaguchi; Takeshi Senga

doi:10.1007/s13277-015-3863-7

UBE2S is associated with malignant characteristics of breast cancer cells

Akter Khondker Ayesha, Toshinori Hyodo, Eri Asano, Naoki Sato, Mohammed A. Mansour, Satoko Ito, Michinari Hamaguchi, Takeshi Senga

Nagoya University

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Ubiquitination is essential for various biological processes, such as signal transduction, intracellular traffick-ing, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskel-eton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.

Original language	English
Pages (from-to)	763-772
Number of pages	10
Journal	Tumor Biology
Volume	37
Issue number	1
DOIs	https://doi.org/10.1007/s13277-015-3863-7 https://doi.org/10.1007/s13277-015-3863-7
Publication status	Published - 6 Aug 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015, International Society of Oncology and BioMarkers (ISOBM).

Keywords

Anoikis
Breast cancer
FAK
Invasion
Migration
UBE2S
Ubiquitination

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "UBE2S is associated with malignant characteristics of breast cancer cells",

abstract = "Ubiquitination is essential for various biological processes, such as signal transduction, intracellular traffick-ing, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskel-eton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.",

keywords = "Anoikis, Breast cancer, FAK, Invasion, Migration, UBE2S, Ubiquitination",

author = "Ayesha, {Akter Khondker} and Toshinori Hyodo and Eri Asano and Naoki Sato and Mansour, {Mohammed A.} and Satoko Ito and Michinari Hamaguchi and Takeshi Senga",

note = "Publisher Copyright: {\textcopyright} 2015, International Society of Oncology and BioMarkers (ISOBM).",

year = "2015",

month = aug,

day = "6",

doi = "10.1007/s13277-015-3863-7",

language = "English",

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journal = "Tumor Biology",

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T1 - UBE2S is associated with malignant characteristics of breast cancer cells

AU - Ayesha, Akter Khondker

AU - Hyodo, Toshinori

AU - Asano, Eri

AU - Sato, Naoki

AU - Mansour, Mohammed A.

AU - Ito, Satoko

AU - Hamaguchi, Michinari

AU - Senga, Takeshi

PY - 2015/8/6

Y1 - 2015/8/6

N2 - Ubiquitination is essential for various biological processes, such as signal transduction, intracellular traffick-ing, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskel-eton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.

AB - Ubiquitination is essential for various biological processes, such as signal transduction, intracellular traffick-ing, and protein degradation. Accumulating evidence has demonstrated that ubiquitination plays a crucial role in cancer development. In this report, we examine the expression and function of ubiquitin-conjugating enzyme E2S (UBE2S) in breast cancer. Immunohistochemical analysis revealed that UBE2S is highly expressed in breast cancer. The depletion of UBE2S by siRNA induced disruption of the actin cytoskel-eton and focal adhesions. Interestingly, phosphorylation of FAK at Tyr397, which is important for the transduction of integrin-mediated signaling, was significantly reduced by UBE2S knockdown. We also show that UBE2S knockdown suppressed the malignant characteristics of breast cancer cells, such as migration, invasion, and anchorage-independent growth. Our results indicate that UBE2S could be a potential target for breast cancer treatment.

KW - Anoikis

KW - Breast cancer

KW - FAK

KW - Invasion

KW - Migration

KW - UBE2S

KW - Ubiquitination

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DO - 10.1007/s13277-015-3863-7

M3 - Article

SN - 1423-0380

VL - 37

SP - 763

EP - 772

JO - Tumor Biology

JF - Tumor Biology

IS - 1

ER -

UBE2S is associated with malignant characteristics of breast cancer cells

Abstract

Bibliographical note

Keywords

UN SDGs

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